#I2511)Introduction. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. #SpectraMax L Config) Methods. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Protein quantitation Cross-talk < 0. Please refer to the Obsolete Microplate Reader Models for more information. A single-mode plate reader is a good starting point. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. a white 96-well half-area microplate (Corning cat. Wavelength Selection. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Documentation:1. 5°C at 37°C. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. absorbance, fluorescence, and luminescence with the added functionality of. 300 µL of Detergent Compatible Bradford reagent was added to each well. Molecular Devices' microplate readers. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. • 0. softmaxt pro microplate data analysis, molecular devices’ stakmax microplate stacker, monochromator-based fluorimeter, wavelength optimization, fda 21 cfr part 11. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. 14: Average %CV. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. i3x. 1 mL/well of media. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Pick the best speed, sensitivity, and price for your needs. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. 76 L x 85. Molecular Devices provides. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Also refer to this Multimode Reader Comparison Chart. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. iD5. Four wash/rinse bottles are included and configurable for both 96- and 384. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. A single-mode plate reader is a good starting point. Plate reader instructions Computer. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. and luminescence microplate reader validation DATA SHEET. Related Products: Low Binding Plates. Please refer to the Obsolete Microplate Reader Models for more information. accurately over a wide dynamic range. Related Products: Low Binding Plates. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Microplate Formats: 6, 12, 24, 48, 96, 384. 3:40 . Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Instrument settings are listed in Table 1. Use standard plate unless you see your type listed. emax and Vmax microplate readers. #PLUS 384) Methods Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Use black microplates. Get. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. In simplest terms, a plate reader measures and. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. ValitaTiter 96-well (Pack of 20). 125 pM. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Click on the crosshair and move it so that it is centered over the well image. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. microplates or in a cuvette at a selected. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 8. 384-well: 0. 0 96 Well Plate. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. We would like to show you a description here but the site won’t allow us. Figure 2. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Microplate reader assays requiring infrared (IR) or red-shifted. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. ©2017 Molecular Devices, LLC . Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). While they play an important role in generating data from a wide range of. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. The plate reader optics are tunable for. Learn more. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. Model:. 384 Awareness Technology ChroMate BioTek Synergy Mx. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Plate wash time (300 µl). It features a modular design that allows users to add or change detection cartridges according to their needs. (Molecular Devices cat. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. 3% in white 96- and. 2 OD at 200 nm. A. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. Listing: #4772697. Microplate Capacity Standard 96-well microplates Incubation Temperature control up to 50ºC Temperature uniformity ± 0. 984. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. • 0. Integrated stacker option. 96-well microplate at 200 µL/well. to each well. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Instrument settings can be saved as a protocol file and used repeatedly for reading differentMolecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. Retrieving. When reading at wavelengths. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. 5. Find a new or used microplate reader and lab equipment for sale and auction at LabX. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. The contents of the SpectraDrop Microplate kit depend on kit type. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 747. Molecular Devices' microplate readers. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Lucid Scientific. Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. , Sigma cat. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Average StDev (mP) 1. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. Microplate Reader, Molecular Devices Optimax, Refurbished. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Shaking: Linear and Orbital. Technical specifications. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. 6 L (with SpectraMax i3x System) Title: SpectraMax MiniMax 300 Imaging Cytometer | Molecular. Molecular. reagent basin for ease of pipetting into. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. 76 L x 85. This reader provides visible. 05. Products : SpectraMax M2/M2e reader, SpectraMax. Microplate Readers. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The Perkin Elmer Wallac 1420. ’s GloMax®-96, for instance, is a dedicated luminometer, whereas Bio-Rad Laboratories’ iMark and xMark strictly measure absorbance. 012 fmol 0. #iD5-STD) Methods ATP standard curve. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. Learn more about how absorbance is measured, and some key applications that utilize. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Both readers were heated to 37ºC prior to setting up the assay. Specifications for the SpectraMax L microplate reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. or ValitaTiter Plus (Molecular Devices cat. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 2 mm x 86 mm) Height:. Published Dec 16, 2020 | Updated Dec 16, 2020. 1700 Online: Sales Offices. 96-well solid bottom, clear polystyrene microplates (Greiner cat. Our microplate. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W (ABS Plus) • Option to run samples as low as 2 μL with the SpectraDrop™ Micro-Volume Microplate • Runs various assays within the Vis/UV-Vis range such asI tried Nunc Edge 2. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. I have used Molecular Devices’ Vmax 96-well microplate readers since about the time of their inception in the mid to late-1980s. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. The microplate reader then generates data following a preconfigured protocol that performs the necessary calculations on the data, delivering consistent results. Omnia® Compatible Microplate Reader Documentation Version No. 03 pM 384-well: 0. 0 OD at. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. OEM Molecular Devices All Microplate Reader, Top r. For more sample throughput, the system canRating: 3. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. We would like to show you a description here but the site won’t allow us. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 8 Pages. About;. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. for ValitaTiter Assays. If the best wavelengths are unknown, the Gemini EM reader will find. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The Molecular Devices Versamax Microplate Reader INCLUDED:. Molecular Devices has provided scientists with . #SpectraMax L Config) Methods. Microplate luminescent reader assay for ERK activity. High tolerance for contaminants. 0 500 1000 1500 2000 2500 3000 3500 time (sec. < 0. #G7294). White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 98 Bovine serum albumin mg/mL O. Place the microplate in the drawer. Optimized for fluorescence microplate readers. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. This is equivalent to having 511 built-in filters,Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. Condition: Used - Good. The system provides options for 20-, 40-,. Flexible for 6-well to 384-well microplates and cuvettes. 0. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. 7°C and the bottom to 36. Transfer of an assay to 96-well microplate technology increases throughput significantly. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The Multiskan Sky microplate reader is an absorbance plate reader. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Measure more accurately. Simple, economical . 04 sec. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Cross-talk < 0. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Published Dec 16, 2020 | Updated Dec 16, 2020. New SpectraMax iD5 Multi-mode Microplate Reader. Fluorescence Microplate Reader Comparison Chart. Jon Cuccui, Brendan W. 98 VMax R2 = 0. Molecular Devices gmbH Tel. 0 OD Photometric resolution: 0. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 2 W x 44. ± 0. #3676). Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Softmax Pro GxP Software. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Detergent Compatible Bradford assay. Step 2: Click the Read button in SoftMax Pro Software. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. DNA concentration (ng/mL) Average RFU. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. flexibility of monochromator-based systems. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. It also integrates with the powerful SoftMax Pro Software for. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. #N207) LAL Reagent Water (Lonza cat. Or scan up to 384 wells at any time to determine the best assay conditions. 1. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. A 1:10 serial dilution of ATP was prepared in medium. Both readers were heated to 37ºC prior to setting up . Materials. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. 03 pM. 19 H mm that meet microplate industry standards. For more sample throughput, both readers can be easily integrated into full roboticVisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath: Datapaq Express (Vial Rack Bottom Label 2D Reader). for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Plates can put in the drawer both in landscape and portrait positions. microplate . #N207) LAL Reagent Water (Lonza cat. 0 H x 60. Paradigm: Delta mP. SOP Title: Use and. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. i3x Multi-Mode Microplate Reader. Wide range of applications The VersaMax reader covers. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. At 5 seconds to read a 96-well endpoint assay, the EMax Microplate Reader has both the speed and performance to carry a heavy workload. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. g. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Published Dec 16, 2020 | Updated Dec 16, 2020. #ID3-STD) L-Tryptophan (Sigma cat. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. Microscopy. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. /well integration in 96-well luminescence mode and 0. 5°C at 37°C. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. 96-well microplate compatible Well read area range: D4 to F11 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-VolumeLearn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. Total volume (except deep. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. A multitude of modalities. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. Table 1: Categories of heating element design. 40266. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. Bioz Stars score: 86/100, based on 1 PubMed citations. The reader adds up to three transfers of assay reagents from a 96- or 384. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Step 2: Launch SoftMax Pro Software. Insert the filled microplate into the drawer, matching well A1 with position A1. As a result, assay flexibility is consequence-free. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® ABS Plus Microplate Reader can run . Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. 0200-6263. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Detergent Compatible Bradford assay. Standard SBS 96 and 384 well plates (128. 2 OD at 200 nm; this decreased to 0.